M-CSF and GM-CSF influence naïve murine alveolar macrophage differentiation and function in vitro Higgins, Jonathan R. "This research has been published in Relative Levels of M-CSF and GM-CSF Influence the Specific Generation of Macrophage Populations during Infection with Mycobacterium tuberculosis by Higgins, D et al. in Journal of Immunology, 2008, 180: 4892-4900." The objective of this study was to compare the effect of M-CSF and GM-CSF in their abilities to affect the functional and phenotypical characteristics of AMs. In this study, we harvested naïve uninfected murine alveolar macrophages by bronchoalveolar lavage (BAL) and cultured in the presence of M-CSF, GM-CSF or both. GM-CSF stimulated cell proliferation at a higher rate when measured by relative loss of CFDA-SE dye and had higher phagocytic capacity than M-CSF cultured cells. In contrast, alveolar macrophages cultured in the presence of M-CSF exhibited a dendritic-cell-like morphology and upregulated expression of dendriticcell- associated markers like CCR7, MHC II and DEC205. The dendritic cell character of M-CSF-treated AMs was also manifested in their higher capacity to stimulate CD4+ T cell proliferation in a mixed lymphocyte reaction (MLR). This study demonstrates that M-CSF has a strong ability to differentiate AMs into DC-like cells. Colorado State University. Libraries Higgins, David M. Lee, Eric J. Orme, Ian M. Gonzalez-Juarrero, Mercedes 2008 Text ; Still Image application/pdf CURC2008_Higgins.pdf STUF2008100003CURC eng English "http://lib.colostate.edu/curc/copyright.html"
M-CSF and GM-CSF influence naïve murine alveolar macrophage differentiation and function in vitro
Higgins, Jonathan R.
"This research has been published in Relative Levels of M-CSF and GM-CSF Influence the Specific Generation of Macrophage Populations during Infection with Mycobacterium tuberculosis by Higgins, D et al. in Journal of Immunology, 2008, 180: 4892-4900."
The objective of this study was to compare the effect of M-CSF and GM-CSF in their abilities to affect the functional and phenotypical characteristics of AMs. In this study, we harvested naïve uninfected murine alveolar macrophages by bronchoalveolar lavage (BAL) and cultured in the presence of M-CSF, GM-CSF or both. GM-CSF stimulated cell proliferation at a higher rate when measured by relative loss of CFDA-SE dye and had higher phagocytic capacity than M-CSF cultured cells. In contrast, alveolar macrophages cultured in the presence of M-CSF exhibited a dendritic-cell-like morphology and upregulated expression of dendriticcell- associated markers like CCR7, MHC II and DEC205. The dendritic cell character of M-CSF-treated AMs was also manifested in their higher capacity to stimulate CD4+ T cell proliferation in a mixed lymphocyte reaction (MLR). This study demonstrates that M-CSF has a strong ability to differentiate AMs into DC-like cells.
Colorado State University. Libraries
Higgins, David M.
Lee, Eric J.
Orme, Ian M.
Gonzalez-Juarrero, Mercedes
2008
Text ; Still Image
application/pdf
CURC2008_Higgins.pdf
STUF2008100003CURC
eng
English
"http://lib.colostate.edu/curc/copyright.html"